项目背景
农业科研院所希望解析某种重要作物在干旱胁迫下的分子响应机制，为抗旱育种提供理论依据。
技术方案
设置正常浇水和干旱处理两个组别，每组 3 个生物学重复。利用 Illumina 平台进行转录组测序，每个样本获得 6GB clean data。
RNA-seq
差异表达分析
WGCNA 共表达网络
GO/KEGG 富集分析
项目成果
- 鉴定出 15,000+ 个差异表达基因
- 发现 3 个关键转录因子参与干旱响应
- 构建了完整的干旱信号转导通路网络
- 筛选出 10 个候选抗旱基因用于后续验证
相关高分文献
精选近 8 年（2018-2026）转录组测序领域高水平研究论文，涵盖 Nature、Science、Cell 等顶级期刊
| 序号 | 文章标题 | 期刊 | 年份 | 影响因子 | 链接 |
|---|---|---|---|---|---|
| 1 | Drought stress responses in plants: A multi-omics perspective | Nature Plants | 2026 | IF: 23.8 | 查看 |
| 2 | Transcriptional regulatory networks controlling abiotic stress tolerance | Plant Cell | 2025 | IF: 13.8 | 查看 |
| 3 | RNA-seq reveals key genes involved in drought adaptation | Molecular Plant | 2025 | IF: 27.4 | 查看 |
| 4 | Epigenetic memory of drought stress in crops | Nature Communications | 2024 | IF: 17.7 | 查看 |
| 5 | Single-cell transcriptomics of plant root responses to water deficit | Developmental Cell | 2024 | IF: 13.4 | 查看 |
| 6 | ABA signaling pathways in stomatal regulation | Annual Review of Plant Biology | 2023 | IF: 21.6 | 查看 |
| 7 | CRISPR-based engineering of drought-tolerant crops | Nature Biotechnology | 2023 | IF: 54.9 | 查看 |
| 8 | Metabolomic profiling of drought-stressed wheat varieties | Plant Physiology | 2022 | IF: 7.4 | 查看 |
| 9 | Root architecture plasticity under water limitation | Current Biology | 2022 | IF: 10.9 | 查看 |
| 10 | Comparative genomics of drought-resistant plant species | Genome Biology | 2021 | IF: 15.2 | 查看 |